Advances in Giardia Research by Peter Malcolm Wallis, Brian R. Hammond

By Peter Malcolm Wallis, Brian R. Hammond

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Bar equals 5 microns. Figures 3 and 4.  The cyst with nonviable morphology (N) seen in Figure 3 was stained with the fluorogenic dye, PI, as shown in Figure 4, and fluoresced orange. Bar equals 5 microns for Figures 3 and 4. Figure 5.  Bar equals 1 micron. Figures 6 and 7. SEMs of a Giardia culture after exposure to bile for three days. Giardia cysts (arrowheads) formed in vitro were seen scattered between flagellated trophozoites, which were attached to the substratum. At higher magnification, seen in Figure 7, the contrast between the flagellated trophozoite and the in vitro formed cyst was readily apparent.

53:371­380.  3, Pergamon Press, Oxford, p 565­718. ), Waterborne Transmission of Giardiasis, Environmental Protection Agency 600/9­79­ 001, p 217­229.  15:26­30.  18:1­26.  A) 148:166­168.  71:89­95.  Med.  15:743­750. 64:165­171.  49:164­171.  44:140­173.  140:222­228.  23:850­855.  549­556.  p 578.  Protozool. 32:383­390.  Gastroenterology 71:57­61.  33:1179­1183.  Microbiol. 45:65­74.  80:49­52.  Parasitologia 4:510­514.  12:851­853.  Science 92:416­417. A.  Cysts formed in vitro were both morphologically and immunologically similar to Giardia cysts formed in vivo and also were viable, as demonstrated by the uptake of fluorogenic dyes.

Gastroenterology 71:57­61.  53:704­707.  J.  73:723­729.  Gastroenterology, In press. 9­21.  lamblia trophozoites.  These observations suggest that direct cell to parasite contact may play an important role in damaging the host cells.  One reported the significance of bile salt deconjugation by the parasite (12) and the other the cytopathogenic effects of Giardia on cell monolayers, in vitro (11).  lamblia on HeLa and Vero cells.  30888 (9) were maintained in Diamond's TPS­1 (4) complete filter sterilized medium according to the method of Visvesvara (14).

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